Department of Means and Methods of Sorption Therapy

nikolaev v-gHead of the Department — Vladimir G. Nikolaev

Corr. Member of the NAS of Ukraine, Prof., Dr.Sci.(Med.)

Laureate of the State Prize of USSR in Science and Technology
Honoured Worker of Science and Technology of Ukraine

Personal page

Tel +38 (044) 257-90-55

E-mail aos@onconet.kiev.ua


Priority areas

  • Fundamental research on the theory of sorption detoxification processes
  • Creation of new medical technologies for withdrawal of toxic substances from the body
  • Development of new approaches to the clinical use of drugs and medical devices

Key words

  • carbon sorbents, hemosorbtion, enterosorption, adsorption therapy, application adsorption therapy
Research interests
  • Development of strong carbon hemosorbents with mass fractal structure designed to effectively remove protein- and membrane-bound metabolites and toxins of different chemical nature, including antitumor cytotoxic agents
  • Development of approaches to optimizing the sorption-related and mechanical characteristics of carbon hemosorbents
  • Development of immunosorbents based on a carbon matrix with immobilized bioactive ligands
  • Development of highly efficient lipid-carbon systems designed for extracting lipotropic substances
  • Developing and exploring new enterosorbents with a wide range of absorbing properties from carbon- and silicon-containing materials
  • Development of the application composite materials combining a carbon sorption component and a biologically active component
  • Study of physico-chemical properties and mechanisms of biological action of hemo-, entero-, and application sorbents, development of test systems for preclinical evaluation
  • Research into the interaction between human serum albumin and its conformers with nanomaterials of different chemical nature
  • Research into structural and functional features of human serum albumin molecules during an oxidative stress
Grants
  1. Seventh Framework EU Programme «MEAD-ET». «The new medical adsorbents for the treatment of life-threatening lesions», No. 247 547, 2011
  2. Seventh Framework EU Programme «Acrobat» Grant agreement No. 286366 FP7-People-2011-IAPP «Carbon adsorbents for the removal of dietary toxins»
  3. Innovative project «Development and optimization of the technology to protect bone marrow from cytostatic myelodepression through integrated use of mass fractal carbon enterosorbents and granulocyte colony-stimulating factor», 2013
Conferences

Publications

Monographs

  1. Николаев В.Г. Энтеросгель. — Киев: «Богдана», 2010. — 159 c.

Informational edition

  1. Краткое наставление по современной тактике применения гемосорбционного метода в клинической практике. ИЭПОР им. Р.Е. Кавецкого НАН Украины, ПИИ ООО «Кыулонг», Киев, 2011.
  2. Методические рекомендации «Доклиническое изучение энтеросорбентов» Минздрав Украины, Гос. экспертный центр МЗ Украины, Киев, 2010.

Articles (Selected)

  1. Sakhno L.A., Yurchenko O.V., Maslenniy V.N., Bardakhivskaya K.I., Nikolaeva V.V., Ivanyuk A.A., Shevchuk O.O., Korotich V.G., Nikolaev V.G. Enterosorption as a method to decrease the systemic toxicity of cisplatin // Exp. Oncology. — 2013. — V. 35, N 1. — P. 45-52.
  2. Sarnatskaya V.V., Sidorenko A.S., Klimchuk D.A., Khohulya I.M., Yushko L.A., Maslenniy V.N., Nikolaev V.G. Optimization of physico–chemical properties of carbon enterosorbents and evaluation of their sorption activity for use in the treatment of paraneoplastic syndrome and other endogenous intoxications in cancer patients // Exp. Oncology. — 2013. — V. 35, N 2. — P. 1-6.
  3. Сарнацкая В.В. Взаимодействие функционализированных алеиновой кислотой и полиэтиленглюколем наночастиц Fe3O4 с альбумином // Доповіді НАН України. — 2013. — No. 9. — С. 164-171.
  4. Мikhalovsky. S.V., Sandeman S.R., Howell C.A., Phillips G.J., Nikolaev V.G. Novel Carbon Adsorbent: In Chapter 21 // Biomedical Applications of Carbon Adsorbents. — First Edition. — 2012. — P. 639-669.
  5. Сарнацкая В. В. Почечная недостаточность: I. Химическая теория патогенеза уремического синдрома // Загальна патологія та патологічна фізіологія. — 2012.– Т. 7, No. 2. — С. 25-31.
  6. Бардахивская К.И., Николаев В.Г., Уваров В.Ю., Цимбалюк Р.С., Иванюк И.И. Иммуносорбция в лечении дилатационной кардиомиопатии // Эфферентная и физико–химическая медицина. — 2012. — No. 3. — С. 7-10.
  7. Shevchuk O.O., Posokhova E.A., Sakhno L.A., Nikolaev V.G. Theoretical ground for adsorptive therapy of anthracyclines cardiotoxicity // Exp. Oncology. — 2012. — V. 34, N 4. — P. 314-322.
  8. Nikolaev V.G., Sakhno L.A., Snezhkova E.A., Sarnatskaya V.V., Yushko L.A., Саrbon adsorbents: achievements and perspectives // Exp. Oncology. — 2011. — V. 33, N 1. — P. 2-8.
  9. Снежкова Е.А., Николаев В.Г., Тридон А., Сарнацкая В.В., Юшко Л.А. О целесообразности создания гепаринсодержащих гемосорбентов // Эфферентная и физико–химическая медицина. — 2011. — No. 2. — C. 3-5.
  10. Бардахивская К.И., Николаев В.Г., Стеблянко Т.И., Кунская Л.Н. Влияние углеродного энтеросорбента на биохимические показатели крови при индуцированной дислипидемии у кролей // Эфферентная и физико–химическая медицина. — 2011. — No. 2. — С. 6-9.
  11. Gun'ko V.M., Turov V.V., Kozynchenko O.P., Nikolaev V.G., Tennison S.R., Meikle S.T., Snezhkova E.A., Sidorenko A.S., Ehrburger–Dolle F., Morfin I., Klymchuk D.O., Mikhalovsky S.V. Activation and structural and adsorption features of activated carbons with highly developed micro–, meso–, and macroporosity // Adsorption. — 2011. — V. 17 (3). — P. 453-460.
  12. Сарнацкая В.В., Николаев В.Г., Михаловский С.В., Юшко Л.А., Сахно Л.А., Масленный В.Н., Николаев А.В., Снежкова Е.А. Делигандизация сывороточного альбумина как новый подход к лечению хронической уремии // Эфферентная и физико–химическая медицина. — 2010. — No. 1. — С. 3-12.
  13. Сахно Л.А., Сарнацкая В.В., Масленный В.Н., Юшко Л.А., Корнеева Л.Н., Коротич В.Г., Николаев В.Г. Сравнительная оценка способности энтеросорбентов различной природы связывать бактериальные эндотоксины // Доповіді НАН України. — 2009. — No. 2. — С. 168-172.
  14. Li Sh., Wu W., Campidelli S., Sarnatskaуa V., Prato M., Tridon A., Nikolaev V., Bianco A., Snezhkova E. Adsorption of carbon nanotubes on active carbon microparticles //Carbon. — 2008. — V. 46. — P. 1091-1109.
  15. Park T.–J., Lee S.–H., Simmons T.J., Martin J.G., Mouza S.A., Snezhkova E.A., Sarnatskaya V.V., Nikolaev V.G., Linhardt R.J. Heparin–cellulose–charcoal composites for drug detoxification prepared using room temperature ionic liquids // Chem.Commun. — 2008. — P. 5022-5024.
  16. Пономарьова О.В., Півнюк В.М., Носко М.М., Сахно Л.О., Дехтяр Т.В., Ніколаєв В.Г., Чехун В.Ф. Профілактика за допомогою вуглецевого ентеросорбенту гострої та відстроченої еметогенної токсичності хіміотерапевтичного лікування онкологічних хворих // Онкология. — 2008. — T. 10, No. 3. — C. 370-373.
Patents

Overall number of patents is 61

Patents obtained in recent years

  1. Utility model patent No. 83399 «A method for producing highly active carbon hemosorbents from carbonizate» dated 10.09.2013
  2. Utility model patent No. 69817 «A method for removal of tryptophan from the blood of patients with malignant tumors» dated 10.05.2012
  3. Utility model patent No. 60251 «A method for evaluating the effectiveness of treatment of patients with chronic hepatitis C» dated 10.06.2011

Certificates of State Registration

The state registration of the developed means for sorption therapy, which is a prerequisite for their use in medical practice has been an integral part of the department's work. Currently, over 20 certificates of state registration have been obtained for sorbents designed for medical use. The most important of these certificates are listed below:

  1. Granular deliganding hemosorbent (GDH) for effective removal of protein-bound toxins during hemosorption, which ensures deep cleaning of proteins and membranes of blood cells (TU 88 Ukraine 258.009-94, Certificate of State Registration No. 9267/2010). Based on GDH, the first domestic technologies «Artificial Liver» 1, 2 and 3 were developed
  2. Ruggedized carbon hemosorbents SKN-1K and SKN-2K in sorption columns not covered with synthetic coal with high biocompatibility, which are designed for the effective removal of endogenous and exogenous toxins and deep cleansing of blood and plasma (TU 88 258.019-2000, Certificate of State Registration No. 3516/2004)
  3. Special nutrient — biologically active supplement to the diet «Carboline». It is used to prevent the emergence and development of the syndrome of endogenous intoxication and somatogenic phase of acute poisoning (TU 24.6-05416946-002-2003, Ministry of Health of Ukraine No. 05.03.02-04/ 66718 dated 16.10. 2008)
  4. Dressings materials based on fibrous carbon materials with well-developed (1500 cm2/g) surface sorption and unique sorption-kinetic characteristics that ensure rapid absorption of a large number of wound contents of different biologically active components which enables their effective use during any phase of wound healing (TU 24.4-05416946-001:2010, Certificate of State Registration No. 9698/2010)
  5. Biospecific carbon hemosorbent CBHS that combines absorptive properties of highly porous activated carbon and acceptor properties of immobilized DNA and heparin, thus ensuring effective removal of blood DNA-binding agents, whose level is significantly increased in a number of pathological conditions. (TU 33.1-05416946-003:2011, Certificate of State Registration No. 9698 /2011)
Innovations

Creating new sorbents for medical use, deep understanding of their mechanisms of action and experimental evaluation of their efficiency makes it possible to expand the range of problems that can be addressed using a sorption therapy.

  1. Granular deliganding hemosorbent (GDH) helps address the key challenge of extracorporeal blood detoxification, namely the problem of efficient absorption of protein-bound membrane-tropic toxins and obtain qualitatively new results in the treatment of a number of serious diseases. Thus, the use of GDH in the treatment of multiple organ failure at late stages of Amanita phalloides poisoning helps reduce child mortality by a factor of 6. An absorption system based GDH is capable of ensuring a powerful sorption barrier, limiting the penetration of cytotoxic drugs into the systemic circulation, thus opening the possibility of regional supply of massive doses of cytostatics in the tumor area without the risk of systemic toxicity.
  2. Carbon biospecifical hemosorbent (CBHS) based on immobilized GDH with immobilized DNA is effective in treating immunodependent diseases, such as severe forms of psoriasis and psoriatic arthritis. GDH with immobilized heparin showed some success in the treatment of dilated cardiomyopathy that develops in the late periods after chemotherapy with anthracycline antineoplastic antibiotics.
  3. A fundamentally new step in the sorption therapy was the creation of the 4th generation of carbon enterosorbents, registered under the brand Carboline. This is a moist bolus or granules formed from carbon fibers or carbon micronized particles and water as a binder. It is proved that carbon chelators can protect the kidney of experimental tumor-bearing animals from nephrotoxic action of cisplatin without reducing the antitumor activity of cytostatics and ensure the normalization of all major elements of lipid and protein metabolisms in rabbits with dyslipidemia. The use of massive doses of Cabroline in patients with moderate to severe forms of El Tor cholera can dramatically reduce the amount of bowel movements and thus retain the required volume of infusions. Administration of Carboline using a special scheme during intensive chemotherapy leads to a 4-fold increase in the percentage of cancer patients with zero degree emetogenic reaction.
  4. Exploring the biomedical mechanisms for the application-sorption treatment of wounds and burns has culminated in the development of a simple and effective method for local detoxification and creating the application sorption dressing based on fibrous carbon sorbents. Its use after surgical removal of the melanoma after prior thermal radiation treatment of the affected areas significantly improves the outcome of autodermoplastics of skin defects. A new way of effective treatment of Grades 2/3 thermal burns based on the application of wet carbon sorbent bandage for 3 days, during the first hour after the injury is very promising.

Staff

The department staff consists of 13 highly skilled employees who perform a wide range of research in the field of sorption detoxication:

  • Member of the National Academy of Sciences of Ukraine, MD
  • 1 Sc.D.
  • 6 PhDs in Biological Sciences
  • 2 PhDs in Technology Science
  • 1 Senior Engineer
  • 2 engineers of category 1
Collaboration

In order to introduce new developments in the clinical practice, the department effectively collaborates with a wide range of Ukrainian medical institutions.

Awards

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